• Handout (PDF)

In addition to the questions listed in the handout, please complete the following:

• Woods Hole MATLAB Tutorial (Zip archive - 112 MB, Caltech only)
  Note: This archive contains two tutorial PDF files (plus supporting images): an introduction/MATLAB overview and an image analysis tutorial. The intro section is optional, but is recommended if you are new to MATLAB
• Cyanobacteria Reading (weblink)
  http://www.genoscope.cns.fr/spip/Synechococcus-ubiquitous-marine.html

• Handout (PDF)
• Protocols for Weeks 1 and 2 (as a reference) (PDF)
• Manual for fixed, fluorescent cell slides (PDF)

• See handout.

• Handout (PDF)
• Brief overview of particle tracking methods (PDF)

• See handout.

• Pre-class exercise with solution (PDF)
• Handout (PDF)
• Arbel's spectroscopy presentation (PPT)
• Jacques Monod - Nobel Lecture - 1965 (PDF)
• Cumming, et al. "Error Bars in Experimental Biology" JCB (2007). (PDF)

• See handout and pre-class exercise.

• Handout (PDF)
• For reference:
  • YFP gene & primer sequences (TXT)
  • In-class molecular biology presentation (PDF)

• See questions in handout.

• Handout (PDF)

• See questions in handout

• Handout (PDF)

• See questions in handout.
• Note: on your molecular biology web page, you also need to write up the procedures you did (to walk through the reader) in addition to the results you obtained--do not just copy the handouts!

• Handout (PDF)
• Bacterial stock listing (XLS)
• Supplemental Reading:
  • Elowitz, et al., Stochastic Gene Expression in a Single Cell, Science 297, 1183-86 (2002)
  • Ozbudak et al., Multistability in the lactose utilization network of Escherichia coli. Nature 2004 Feb 19 427(6976):737-40
  • Müller et al., Repression of lac Promoter as a Function of Distance, Phase and Quality of an Auxiliary lac Operator. J. Mol. Biol. 257: 21-29 (1996)
  • Yagil and Yagil, On the Relation between Effector Concentration and the Rate of Induced Enzyme Synthesis. Biophys J. 1971 January; 11(1): 11–27.