Bi 1x

General

 

 

Schedule

 

Please note: this schedule is tentative and may change as the term progresses.

Week 1

Reading:
Session 1: Logistics and laboratory basics
Pre lab:
  • Read the handout (PDF)

In lab:

 

 
  • Introduction on laboratory techniques
    • Lab safety rules
    • Pipetting
    • "Cell management" - sterile technique and plating
    • Biological spectroscopy using a spectrophotometer
Session 2: Microscopy and Powers of Ten
Pre lab:
  • Read the handout (PDF)

In lab:

 
Session 3: Matlab Tutorial
Pre lab:
  • Show up with your labtops with Matlab installed
In lab:
  • Matlab handout (ZIP)
  • Advanced Matlab handout (ZIP)
  • How to connect to snowdome (PDF)
Schedule
Mon. (4/2)
  • Group 1: Logistics and laboratory basics
  • Group 2: Microscopy and Powers of Ten
Tue. (4/3)
  • Group 1: Microscopy and Powers of Ten
  • Group 2: Logistics and laboratory basics
Thurs. (4/5)
  • Meet at the BBB ponds at 7:00PM
  • Journal club on Nurse paper
  • Matlab Tutorial

Week 2

Reading:
Session 1: Growth Curves
Pre lab:
In lab:
  • Handout UPDATED (PDF)
Session 2: Growth Movies
Pre lab:
In lab:
  • Handout (PDF)
Session 3: Dictyostelium
Pre lab:
  • Read the handout (coming soon!)
  • Write pre-lab in your notebook following the guidelines in the Grading handout (PDF)
In lab:
  • Handout (coming soon!)
  • Journal club on Whitman et al.
Schedule
Mon. (4/8)
  • Group 1: Growth Curves
  • Group 2: Growth Movies
Tue. (4/9)
  • Group 1: Growth Movies
  • Group 2: Growth Curves
Thu. (4/11)
  • Group 1 ONLY: Dictyostelium (woohoo!)
  • Journal club on Whitman et al.
Assignment 1
Due Fri. (4/20)

Week 3

Session 1: Matlab Session
Pre lab:
  • Bring your computers
In lab:
  • Advanced Matlab handout (ZIP)
Session 2: DNA Extraction
Pre lab:
In lab:
  • Protocol (PDF)
Session 3: Dictyostelium
Pre lab:

  
In lab:
  • Handout (coming soon!)
  • Journal club on Whitman et al.
Schedule
Mon. (4/16)
  • Everyone: Matlab tutorial
  • Everyone: Lecture on DNA science
Tue. (4/17)
  • Everyone: DNA extractions
Thu. (4/19)
  • Group 1 ONLY: Dictyostelium (woohoo!)
  • Journal club on Whitman et al.

Week 4

Reading:
Session 1: Bulk restriction digest
Pre lab:
In lab:
  • Protocol (PDF)
Session 2: PCR/Gels
Pre lab:
In lab:
  • Protocol (PDF)
  • Protocol (PDF)
Schedule
Mon. (4/23)
  • Introduction on modern biology
  • Journal club on Van Oijen paper
  • Bulk restriction digest
Tue. (4/24)
  • PCR
  • Gels
Assignment 2
Due Fri. (5/4)

Week 5

Reading:
Session 1: Single Molecule Digest
Pre lab:
  • Read the handout
  • Write pre-lab in your notebook
In lab:
  • Protocol (PDF)
Session 2: Journal Club / PCR Gels
Pre lab:
  • Read the journal paper
  • Write pre-lab in your notebook
In lab:
  • Protocol (PDF)
Schedule
Mon. (4/30)
  • Group 1: Single Molecule Digest
  • Group 2:
    • Journal Club
    • PCR Gels
Tue. (5/1)
  • Group 1:
    • Journal Club
    • PCR Gels
  • Group 2: Single Molecule Digest
Assignment 3
Due Fri. (5/11)

Week 6

Reading:
Session 1: TOPO Cloning
Pre lab:
  • Read the handout
  • Write the pre-lab in your notebook
In lab:
  • Protocol (PDF)
Session 2: Lecture/Journal Club
Pre lab:
  • Read the assigned papers
Schedule
Mon. (5/7)
  • Everyone: TOPO Cloning
Tue. (5/8)
  • Everyone: Lecture/Journal Club
Assignment 4
Due Fri. (5/18)
  • Assignment 4 (will be posted soon)